关键词: 原发性肝癌, 细胞周期蛋白B1, 癌症基因组图谱（TCGA）

Abstract:

objective  To investigate the expression of CyclinB1 (CCNB1) in primary hepatocellular carcinoma tissues and to evaluate its association with clinical pathological features. Methods  The mRNA expression data of CCNB1 and its clinical information were downloaded from The Cancer Genome Atlas (TCGA). Then the relationship between the mRNA expression of CCNB1 and its clinical pathological parameters was analyzed. Cox regression model was performed for the univariate and multivariate analysis of the factors affecting the prognosis of primary hepatocellular carcinoma. GO and KEGG pathway analysis of CCNB1 co-expressed genes were performed. Results  The mRNA expression level of CCNB1 in primary hepatocellular carcinoma was significantly higher than that in normal liver tissue (P<0.001). Moreover, its expression level was significantly correlated with race (P=0.005), AFP (P<0.0001), histological grade (P<0.0001), vascular invasion (P<0.0001) of patients. Kaplan-Meier survival analysis showed that the overall survival (P=0.0015) rate and disease-free survival rate (P=0.0004) in patients with CCNB1 high-expression was significantly lower than that in patients with CCNB1 low-expression. Univariate and multivariate analysis revealed that CCNB1 expression was independent prognostic indicator for poor OS (HR 1.544, 95%CI 1.052~2.266, P=0.027) and DFS (HR 1.793, 95%CI 1.277~2.517, P=0.001). GO and KEGG pathway analysis showed that CCNB1 co-expressed genes were associated with cell proliferation, cell cycle, G1/S transition of mitotic cell cycle, G2/M transition of mitotic cell cycle, p53 signaling pathway. Conclusion  CCNB1 is aberrantly expressed in primary hepatocellular carcinoma and its expression may be a diagnostic biomarker.

Key words: primary hepatocellular carcinoma, CyclinB1(CCNB1), The Cancer Genome Atlas (TCGA)